ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
Specificité
IgG (H&L)
Attributs du produit
Anti-Mouse IgG whole molecule antibody generated in donkey detects specifically Mouse IgG whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
Immunogène
Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in donkey.
Suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other peroxidase antibody-based enzymatic assays requiring lot-to-lot consistency.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Restore with deionized water (or equivalent)
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Agent conservateur
Gentamicin sulfate
Conseil sur la manipulation
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C
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Schorch, Heni, Zahaf, Brummer, Mione, Schmidt, Papatheodorou, Aktories: "Targeting oncogenic Ras by the Clostridium perfringens toxin TpeL." dans: Oncotarget, Vol. 9, Issue 23, pp. 16489-16500, (2018) (PubMed).
Klaric, Thomas, Dottori, Leong, Koblar, Lewis: "A reduction in Npas4 expression results in delayed neural differentiation of mouse embryonic stem cells." dans: Stem cell research & therapy, Vol. 5, Issue 3, pp. 64, (2015) (PubMed).
Hemmasi, Czulkies, Schorch, Veit, Aktories, Papatheodorou: "Interaction of the Clostridium difficile Binary Toxin CDT and Its Host Cell Receptor, Lipolysis-stimulated Lipoprotein Receptor (LSR)." dans: The Journal of biological chemistry, Vol. 290, Issue 22, pp. 14031-44, (2015) (PubMed).
Litjens, Nguyen, Castro, Aromataris, Jones, Barritt, Rychkov: "Phospholipase C-gamma1 is required for the activation of store-operated Ca2+ channels in liver cells." dans: The Biochemical journal, Vol. 405, Issue 2, pp. 269-76, (2007) (PubMed).
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