Anti-Rabbit IgG antibody generated in donkey detects specifically rabbit IgG. This secondary antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Concentration Definition: by UV absorbance at 280 nm
Purification
Preadsorption: Solid phase absorption
Stérilité
Sterile filtered
Immunogène
Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Anti-Rabbit IgG (H&l) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Stock
4 °C
He, Qiu, Dai, Fang, Sun, Gong, Ding, Sun: "MicroRNA-351 inhibits denervation-induced muscle atrophy by targeting TRAF6." dans: Experimental and therapeutic medicine, Vol. 12, Issue 6, pp. 4029-4034, (2016) (PubMed).
Hua, Han, Zhou, Kim, Shi, Abeysirigunawardena, Jain, Singh, Aggarwal, Woodson, Ha: "An improved surface passivation method for single-molecule studies." dans: Nature methods, Vol. 11, Issue 12, pp. 1233-6, (2015) (PubMed).