Chèvre anti-Lapin IgG (F(ab')2 Region) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

N° du produit ABIN102041

Détail du produit

Antigène: IgG

Épitope: F(ab')2 Region

Reactivité: Lapin

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Alkaline Phosphatase (AP)

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: IgG F(ab')2

Attributs du produit: Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Stérilité: Sterile filtered

Immunogène:

Immunogen: Rabbit IgG F(ab')2 fragment

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:200 - 1:1,000

Application Note: This product has been assayed against 1.0 μg of Rabbit IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:2,000 - 1:10,000

Western Blot Dilution: 1:500 - 1:2,500

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.75 mg/mL

Buffer:

Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed.

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Goat Anti-Rabbit IgG F(ab')2 Antibody alkaline phosphatase Conjugation, Goat Anti-Rabbit IgG Fab2 alkaline phosphatase Conjugated Antibody, Goat Anti-Rabbit IgG Fab2 Fragment alk phos Conjugated Antibody

Background: Anti-Rabbit IgG F(ab')2 Antibody generated in goat recognizes the dimeric Fab portion of the rabbit IgG molecule. Rabbit IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind rabbit IgG F(c) will not bind rabbit IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.