Chèvre anti-Rat IgG (Heavy & Light Chain) Anticorps - Preadsorbed

N° du produit ABIN102117

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Rat

Hôte: Chèvre

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: IgG (H&L)

Attributs du produit: Anti-RAT IgG (H&L) (GOAT) Antibody generated in sheep detects specifically rat IgG whole molecule. Anti-rat IgG antibody is ideal for investigators involved in serum protein component research.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: immunoaffinity chromatography using Rat IgG coupled to agarose beads

Stérilité: Sterile filtered

Immunogène:

Immunogen: Rat IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000-1:5,000

Application Note: Anti-RAT IgG (H&L) (GOAT) Antibody is suitable for ELISA, Western Blot and Immunohistochemistry applications. Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:20,000-1:100,000

Western Blot Dilution: 1:2,000-1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Date de péremption: 12 months

Référence

Scekic-Zahirovic, Oussini, Mersmann, Drenner, Wagner, Sun, Allmeroth, Dieterlé, Sinniger, Dirrig-Grosch, René, Dormann, Haass, Ludolph, Lagier-Tourenne, Storkebaum, Dupuis: "Motor neuron intrinsic and extrinsic mechanisms contribute to the pathogenesis of FUS-associated amyotrophic lateral sclerosis." dans: Acta neuropathologica, Vol. 133, Issue 6, pp. 887-906, (2017) (PubMed).

Zhang, Fu, Chen, Ren, Qu, Zhang, Yao, Yang: "Lacidipine attenuates apoptosis via a caspase-3 dependent pathway in human kidney cells." dans: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 32, Issue 4, pp. 1040-9, (2014) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Goat anti-rat IgG, Rat antibody, rat antibodies, immunoglobulin

Background: Anti-RAT IgG (H&L) (GOAT) Antibody generated in goat detects specifically rat IgG whole molecule. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-rat IgG antibody is ideal for investigators involved in serum protein component research.