Chèvre anti-Rat IgG (Heavy & Light Chain) Anticorps (FITC) - Preadsorbed
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- Antigène Tous les produits IgG
- IgG
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Épitope
- Heavy & Light Chain
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Reactivité
- Rat
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- FITC
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Application
- Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
- Specificité
- IgG (H&L)
- Attributs du produit
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- Preadsorption: Solid phase absorption
- Immunogène
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Immunogen: Rat IgG whole molecule
- Isotype
- IgG
- Labeling Ratio
- 3.1
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- Indications d'application
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Application Note: Rat IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000 - 1:50,000
Flow Cytometry Dilution: 1:500 - 1:2,500
IF Microscopy Dilution: 1:1,000 - 1:5,000
- Commentaires
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Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 1.0 mL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
- Product is photosensitive and should be protected from light.
- Stock
- RT,4 °C,-20 °C
- Date de péremption
- 12 months
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: Goat Anti-Rat IgG Fluorescein Conjugated Antibody, Goat Anti-Rat IgG Antibody FITC Conjugation
Background: Anti-Rat IgG (H&L) Antibody Fluorescein Conjugated generated in goat detects reactivity to Rat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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