Lapin anti-Mouton IgG (Heavy & Light Chain) Anticorps (HRP) - Preadsorbed

N° du produit ABIN102262

N° du produit (Fournisseur): 613-4328

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Mouton

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

N° du produit (Fournisseur): 613-4328

Fournisseur: Rockland

Fonction: Sheep IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Réactivité croisée (Details): Minimal crossreactivity against Human Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, Sheep IgG and Sheep Serum.  No reaction was observed against Human Serum Proteins.

Attributs du produit: Anti-Sheep IgG F(ab')2 Antibody generated in rabbit recognizes the dimeric Fab portion of the sheep IgG molecule. Sheep IgG F(ab')2 is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme pepsin under controlled conditions of temperature, time and pH . F(ab')2 Molecules lack the Fc portion of IgG and therefore receptors that bind sheep IgG F(c) will not bind sheep IgG F(ab')2 Molecules. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Sheep IgG F(ab')2 is conjugated to Peroxidase.

Purification: Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogène: Optional[Immunogen]: Sheep IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application: Application Note: Anti-Sheep IgG Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays. Immunohistochemistry Dilution: 1:300 - 1:1,500 Western Blot Dilution: 1:500 - 1:3,000 ELISA Dilution: 1:5,000 - 1:22,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Date de péremption: 12 months

Référence

Bissoon-Haqqani, Moyana, Jonker, Maroun, Birnboim: "Nuclear expression of thymidylate synthase in colorectal cancer cell lines and clinical samples." dans: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 54, Issue 1, pp. 19-29, (2006) (PubMed).

Cripe, Gelfanov, Smith, Spigel, Phillips, Gabig, Jung, Fyffe, Hartman, Kneebone, Mercola, Burgess, Boswell: "Role for c-jun N-terminal kinase in treatment-refractory acute myeloid leukemia (AML): signaling to multidrug-efflux and hyperproliferation." dans: Leukemia, Vol. 16, Issue 5, pp. 799-812, (2002) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet: HRP Conjugated Secondary Antibody is designed for western blotting, ELISA and Immunohistochemistry. Horseradish peroxidase conjugated antibodies are ideal for antibody detection assays that require lot-to-lot consistency.