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Lapin anti-Souris IgG2a (Heavy Chain) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
N° du produit ABIN102374
  • Antigène Tous les produits IgG2a
    IgG2a
    Épitope
    • 50
    • 15
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    Heavy Chain
    Reactivité
    • 101
    • 18
    • 1
    Souris
    Hôte
    • 57
    • 28
    • 17
    • 13
    • 5
    Lapin
    Clonalité
    • 71
    • 30
    Polyclonal
    Conjugué
    • 22
    • 13
    • 13
    • 10
    • 8
    • 5
    • 5
    • 5
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Alkaline Phosphatase (AP)
    Application
    • 57
    • 50
    • 37
    • 32
    • 24
    • 19
    • 11
    • 10
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Specificité
    IgG2a
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    Preadsorption: Solid phase absorption
    Stérilité
    Sterile filtered
    Immunogène

    Immunogen: Mouse IgG2a heavy chain

    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry Dilution: 1:200 - 1:1,000

    Application Note: This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code # NPP-10 as a substrate for 30 minutes at room temperature.  A working dilution of 1:1,000 to 1:5,000 is suggested for this product.

    ELISA Dilution: 1:2,000 - 1:10,000

    Western Blot Dilution: 1:500 - 1:2,500

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.1 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
    Do not add Sodium azide.
    Dilute only prior to immediate use
    Each reagent is stable for the period shown on the bottle label if stored as directed.
    Stock
    4 °C
    Date de péremption
    12 months
  • Antigène
    IgG2a
    Abstract
    IgG2a Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Rabbit Anti-Mouse IgG2a (gamma 2a chain) Antibody alkaline phosphatase Conjugated, Rabbit Anti-Mouse IgG2a Antibody alk phos Conjugation

    Background: Anti-Mouse IgG2a Alkaline Phosphatase antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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