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Lapin anti-Humain IgM (Heavy Chain) Anticorps (FITC)

FACS, FLISA, FM Polyclonal IgG FITC
N° du produit ABIN102634
  • Antigène Tous les produits IgM
    IgM
    Épitope
    • 162
    • 122
    • 87
    • 57
    • 26
    • 9
    • 7
    • 3
    • 1
    • 1
    Heavy Chain
    Reactivité
    • 226
    • 159
    • 89
    • 42
    • 34
    • 34
    • 28
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 11
    • 4
    • 2
    • 1
    Humain
    Hôte
    • 365
    • 230
    • 89
    • 21
    • 14
    • 5
    • 4
    • 1
    • 1
    Lapin
    Clonalité
    • 533
    • 95
    Polyclonal
    Conjugué
    • 122
    • 85
    • 84
    • 66
    • 34
    • 32
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    FITC
    Application
    • 377
    • 282
    • 261
    • 221
    • 130
    • 124
    • 91
    • 91
    • 63
    • 61
    • 53
    • 53
    • 45
    • 38
    • 30
    • 20
    • 18
    • 12
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    Specificité
    IgM µ chain
    Attributs du produit
    Concentration Definition: by UV absorbance at 280 nm
    Purification
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Human IgM and Human Serum.  No reaction was observed against Human IgG.
    Immunogène

    Immunogen: Human IgM mu heavy chain

    Isotype
    IgG
    Labeling Ratio
    4.3
  • Indications d'application

    Application Note: Anti-Human IgM (mu heavy chain) Fluorescein is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:10,000 - 1:50,000

    Flow Cytometry Dilution: 1:500 - 1:2,500

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Commentaires

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 2.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    10.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Product is photosensitive and should be protected from light.
    Stock
    RT,4 °C,-20 °C
    Date de péremption
    12 months
  • Antigène
    IgM
    Abstract
    IgM Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: rabbit anti-Human IgM (mu chain) Fluorescein Conjugated Antibody, rabbit anti-Human IgM mu chain FITC Conjugated Antibody, rabbit anti-Human IgM mu chain Antibody Fluorescein Conjugation, rabbit anti-Human IgM (mu chain) Antibody FITC Conjugation

    Background: Anti-Human IgM (mu heavy chain) Fluorescein generated in rabbit detects specifically Human IgM mu heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. Anti-Human IgM mu chain antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.

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