Lapin anti-Rat IgM (Heavy Chain) Anticorps (Biotin) - Preadsorbed

N° du produit ABIN102713

Détail du produit

Antigène: IgM

Épitope: Heavy Chain

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: IgM µ chain
Rat IgM antibody recognizes the mu chain of the Rat IgM. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum, Rat IgM and Rat Serum. ELISA was used to confirm specificity at less than 1 % cross reactivity against other rat heavy or light chain isotypes.

Attributs du produit: Anti-Rat IgM antibody is ideal with investigators involved in serum component protein research.
Concentration Definition: by UV absorbance at 280 nm

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Rat IgM whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-RAT IgM (mu chain) Antibody has been assayed against Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin.  A working dilution of 1:15,000 to 1:70,000 of the  concentration is suggested for this product. Rat IgM antibody is suitable for immunoassays including western blot and ELISA.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Aliquot to Avoid repeated freezing and thawing.

Stock: RT,4 °C,-20 °C

Date de péremption: 12 months

Détail du antigène

Antigène: IgM

Abstract: IgM Produits

Classe de substances: Antibody

Sujet:

Synonyms: Rabbit Anti-Rat IgM (mu chain) Antibody biotin Conjugated, Rabbit Anti-Rat IgM mu Antibody BAC Conjugation

Background: Anti-Rat IgM antibody generated in rabbit specifically detects rat IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together, the approximate molecular weight of IgM is 900 kDa and possesses 10 binding sites (though due to the size of most antigens, not all sites are capable of binding at once). Due to this large size, IgM is typically isolated to the serum. Anti-Rat IgM antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology. This Anti-Rat IgM antibody is conjugated to biotin.