Âne anti-Poulet IgG (Heavy & Light Chain) Anticorps

N° du produit ABIN1043929

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Poulet

Hôte: Âne

Clonalité: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgG and Chicken Serum.

Réactivité croisée: Poulet

Réactivité croisée (Details): Pre-Adsorbed

Attributs du produit: Chicken IgY antibody generated in donkey detects specifically chicken IgG. This anti-Chicken secondary antibody is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.

Purification: Anti-Chicken IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Chicken IgG and Chicken Serum.   No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Chicken IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-Chicken IgG (H&L) is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.12 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid cycles of freezing and thawing.
Centrifuge product if not completely clear after standing at room temperature.
Dilute only prior to immediate use.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Donkey anti-Chicken IgG Antibody, Donkey anti-Chicken IgG Pre-adsorbed Antibody

Background: Anti-Chicken IgG Antibody generated in donkey detects chicken IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.