Lapin anti-Chévre IgG (Heavy & Light Chain) Anticorps (Atto 655) - Preadsorbed
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- Antigène Tous les produits IgG
- IgG
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Épitope
- Heavy & Light Chain
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Reactivité
- Chévre
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- Atto 655
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Application
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Specificité
- Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-AdsorbedAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Goat IgG and Goat Serum.
- Attributs du produit
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Anti-Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-Adsorbed is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Anti-Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-Adsorbed is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. - Purification
- Preadsorption: Solid phase absorption
- Immunogène
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Immunogen: Goat IgG whole molecule
- Isotype
- IgG
- Labeling Ratio
- 3.5
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- Indications d'application
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Application Note: Anti-Goat IgG (H&L) Antibody ATTO 655 Conjugated Pre-Adsorbed is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- Commentaires
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The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Avoid cycles of freezing and thawing.
Product is photosensitive and should be protected from light. - Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
- Date de péremption
- 12 months
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: rabbit anti-Goat IgG ATTO 655 Conjugated Antibody, rabbit anti-Goat IgG Antibody ATTO655 Conjugation
Background: Anti-Goat IgG ATTO dye Antibody generated in rabbit detects goat IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.
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