Chèvre anti-Hamster arménien IgG (Heavy & Light Chain) Anticorps (Alkaline Phosphatase (AP)) - Preadsorbed
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- Antigène Tous les produits IgG
- IgG
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Épitope
- Heavy & Light Chain
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Reactivité
- Hamsters dorés syriens, Hamster arménien
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- Alkaline Phosphatase (AP)
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificité
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Armenian/Golden Syrian Hamster IgG and Armenian/Golden Syrian Hamster Serum.
- Purification
- Preadsorption: Solid phase absorption
- Stérilité
- Sterile filtered
- Immunogène
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Immunogen: Armenian and Golden Syrian Hamster IgG, whole molecule
- Isotype
- IgG
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- Indications d'application
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Immunohistochemistry Dilution: 1:200-1:1000
Application Note: Anti-Golden Syrian & Armenian Hamster IgG Alk Phos conjugate is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
ELISA Dilution: 1:2000-1:10,000
Western Blot Dilution: 1:500-1:3000
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
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Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Conseil sur la manipulation
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Stock
- 4 °C
- Stockage commentaire
- Store vial at 4 °C prior to restoration. This product is stable for several weeks at 4 °C as an undiluted liquid.
- Date de péremption
- 12 months
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- Antigène
- IgG
- Abstract
- IgG Produits
- Classe de substances
- Antibody
- Sujet
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Synonyms: goat anti-Golden Syrian & Armenian Hamster IgG alkaline phosphatase conjugated antibody, goat anti-Hamster IgG alk phos conjugated antibody
Background: Anti-Golden Syrian & Armenian Hamster IgG Alkaline Phosphatase Antibody generated in goat detects Golden Syrian & Armenian Hamster IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
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