ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Dot Blot (DB), Immunoelectron Microscopy (IEM)
Specificité
Fab Goat IgG (H&L) Antibody Peroxidase ConjugatedAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Donkey Serum.
Attributs du produit
Anti-Goat IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically goat IgG. This secondary peroxidase conjugated antibody anti-Goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Goat IgG (H&L) peroxidase conjugated antibody generated in donkey detects specifically goat IgG. This secondary peroxidase conjugated antibody anti-Goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Purification
Immunoaffinity Chromatography.
Immunogène
Anti-Goat IgG (H&L) was produced by repeated immunization with goat whole molecule in donkey. Immunogen Type: Native Protein
Antibody Anti-Goat IgG (H&L) peroxidase conjugated is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Agent conservateur
Gentamicin sulfate
Conseil sur la manipulation
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Centrifuge product if not completely clear after standing at room temperature.
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.