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Chèvre anti-Souris IgG2a (Heavy Chain) Anticorps (TRITC) - Preadsorbed

IHC, WB, FLISA, FM Polyclonal IgG TRITC
N° du produit ABIN1118477
  • Antigène Tous les produits IgG2a
    IgG2a
    Épitope
    • 50
    • 15
    • 7
    • 2
    • 2
    • 2
    • 1
    • 1
    Heavy Chain
    Reactivité
    • 101
    • 18
    • 1
    Souris
    Hôte
    • 56
    • 29
    • 17
    • 13
    • 5
    Chèvre
    Clonalité
    • 71
    • 30
    Polyclonal
    Conjugué
    • 22
    • 13
    • 13
    • 10
    • 9
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    TRITC
    Application
    • 58
    • 50
    • 37
    • 32
    • 23
    • 18
    • 11
    • 10
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
    Specificité
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2a. Specificity was confirmed by ELISA at less than 1 % cross-reactivity against other mouse heavy or light chain isotypes.
     Réactivité croisée
    Souris
    Attributs du produit
    Mouse IgG2a secondary antibody is available in a variety of formats. Rhodamine secondaaries are designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Mouse IgG2a heavy chain

    Immunogen Type: Native Protein

    Isotype
    IgG
    Labeling Ratio
    3.1
  • Indications d'application

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: Anti-Mouse IgG2a secondary antibody is suitable for ELISA, Immunohistochemistry western blotting as well as other anti IgG2a antibody based assays. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

    FLISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    IF Microscopy Dilution: 1:1,000 - 1:5,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Avoid cycles of freezing and thawing.
    Product is photosensitive and should be protected from light.
    Centrifuge product if not completely clear after standing at room temperature.
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Date de péremption
    12 months
  • Antigène
    IgG2a
    Abstract
    IgG2a Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Goat anti-mouse IgG2a antibody rhodamine conjugation, goat anti-mouse IgG2a (gamma 2a) TRITC conjugated antibody

    Background: Anti-Mouse IgG2a Antibody generated in goat detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

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