ELISA, IHC, WB, DB
Polyclonal
Alkaline Phosphatase (AP)
Indications d'application
Suitable for Immunoblotting (Western or Dot blot) (1/1,000-1/5,000), ELISA (1/10,000-1/50,000), Immunoperoxidase electron microscopy and Immunohistochemistryas well as other peroxidase-antibody based enzymatic assays requiring lot-to-lotconsistency.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Reconstitution
Restore with 1.0 mL of deionized water (or equivalent).
Concentration
1.0 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer and 0.01 % (w/v) Thimerisol as preservative.
Agent conservateur
Thimerosal (Merthiolate)
Précaution d'utilisation
Biohazard Informations: This product contains thimerosal which is hazardous.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C/-20 °C
Stockage commentaire
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.