ELISA: 1:10,000 - 1:100,000. Western Blot: 1:1000 - 1:10,000. Immunohistochemistry on frozen sections: 1:200 - 1:500.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS containing 0.09 % ProclinTM300 and 0.2 % Bovine Serum Albumin
Agent conservateur
ProClin
Précaution d'utilisation
This product contains ProClinTM: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.