FACS, ELISA
Polyclonal
IgG
Biotin
F(ab')2 fragment
Indications d'application
Applications: ELISA - Quality tested FLISA - Quality tested FC - Quality tested , IHC-FS - Reported in literature , IHC-PS - Reported in literature , IP - Reported in literature , Sep - Reported in literature , Costim - Reported in literature
Working Dilutions: ELISA Purified (UNLB) antibody 1 g/mL AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 20,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Agent conservateur
Without preservative
Conseil sur la manipulation
Dilute only prior to immediate use Each reagent is stable for the period shown on the bottle label if stored as directed.