Applications: ELISA - Quality tested , FLISA - Quality tested FC - Reported in literature , IHC-FS - Reported in literature , IHC-PS - Reported in literature , ICC - Reported in literature , WB - Reported in literature , Microarray - Reported in literature , Multiplex - Reported in literature , Purification - Reported in literature
Working Dilutions: ELISA AP conjugate 1:1,000 - 1:2,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:10,000 FLISA FITC, AF488, and AF555 conjugates 1:200 - 1:400 PE and AF647 conjugates ≤ 1 g/mL
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Agent conservateur
Without preservative
Conseil sur la manipulation
Dilute only prior to immediate use Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Stockage commentaire
Store at 2-8°C
Gao, Moore, Porcheray, Rong, Abidoglu, DeVito, Paine, Girouard, Saidman, Schoenfeld, Levin, Wong, Elias, Schuetz, Rosales, Fu, Zorn: "Pretransplant IgG Reactivity to Apoptotic Cells Correlates With Late kidney Allograft Loss." dans: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, Vol. 14, Issue 7, pp. 1581-91, (2014) (PubMed).
Xu, Wang, Deng, Hu, Li, Chen, Ning, Wu, Huang, Li, Wu, Yu, Li: "The serological diagnosis of human clonorchiasis by time-resolved fluoroimmunoassay based on GST2-specific IgG4 detection." dans: Parasitology research, (2013) (PubMed).
Harada, Shimoda, Kimura, Sato, Ikeda, Igarashi, Ren, Sato, Nakanuma: "Significance of IgG4-positive cells in extrahepatic cholangiocarcinoma: Molecular mechanism of IgG4 reaction in cancer tissue." dans: Hepatology (Baltimore, Md.), (2012) (PubMed).
Jackson, Lucas, Melancon, Desai: "Clinical relevance and IgG subclass determination of non-HLA antibodies identified using endothelial cell precursors isolated from donor blood." dans: Transplantation, Vol. 92, Issue 1, pp. 54-60, (2011) (PubMed).
Xu, Chen, Zhao: "High potential to reverse the inhibition of myeloperoxidase by ceruloplasmin of anti-myeloperoxidase autoantibodies of IgG3 subclass." dans: Autoimmunity, (2011) (PubMed).
Xu, Chen, Cui, Zhao: "Influence of myeloperoxidase by anti-myeloperoxidase antibodies and its association with the disease activity in microscopic polyangiitis." dans: Rheumatology (Oxford, England), Vol. 49, Issue 11, pp. 2068-75, (2010) (PubMed).