Souris anti-Humain IgG (Fc Region) Anticorps

N° du produit ABIN1607856

Détail du produit

Antigène: IgG

Épitope: Fc Region

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: ELISA, Western Blotting (WB)

Specificité: IgG F(c)

Réactivité croisée: Humain

Purification: Anti-Human IgG F(c) Antibody was prepared from concentrated roller bottle supernatant by Protein A chromatography sepharose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, Human IgG, Human Serum and Human F(c).  No reaction was observed against Human F(ab).

Immunogène:

Immunogen: Anti-Human IgG F(c) monoclonal antibody was produced by repeated immunization with Human IgG F(c) fragment in mice.

Immunogen Type: Native Protein

Clone: 3D8-H7-D4

Isotype: IgG1 kappa

Information d'application

Indications d'application:

Application Note: Human IgG F(c) antibody is suitable for immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.

ELISA Dilution: 1-:1000-1:10000

Western Blot Dilution: 1:1000 - 1:2000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid cycles of freezing and thawing.
Do NOT add Sodium Azide!
Centrifuge product if not completely clear after standing at room temperature.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 4 °C as an undiluted liquid.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Human IgG F(c) Antibody, Human IgG Fc Antibody, Ms-a-Human IgG Fc, Human IgG F(c) Antibody in Mouse, Human Secondary Antibody

Background: Anti-Human IgG F(c) generated in mouse detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.