Chèvre anti-Souris IgG (F(ab')2 Region) Anticorps (PE) - Preadsorbed

N° du produit ABIN2669886

Détail du produit

Antigène: IgG

Fragment: F(ab')2 fragment

Épitope: F(ab')2 Region

Reactivité: Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: PE

Application: Western Blotting (WB), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Specificité: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum.

Réactivité croisée: Souris

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Mouse IgG F(ab')2 fragment

Isotype: IgG

Information d'application

Indications d'application:

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

Flow Cytometry Dilution: 1:100 - 1:250

Western Blot Dilution: 1:100 - 1:500

IF Microscopy Dilution: 1:100 - 1:250

Commentaires:

Post Translational Modification: Phosphorylation

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20 °C or below after dilution.

Stock: RT,4 °C

Date de péremption: 6 months

Référence

Rappa, Anzanello, Lorico: "Ethanol induces upregulation of the nerve growth factor receptor CD271 in human melanoma cells via nuclear factor-κB activation." dans: Oncology letters, Vol. 10, Issue 2, pp. 815-821, (2015) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: Goat F(ab')2 Anti-Mouse IgG F(ab')2 Antibody phycoerythrin Conjugation, Goat Fab2 Anti-Mouse IgG Fab2 PE Conjugated Antibody

Background: F(ab')2 Anti-Mouse IgG F(ab')2 Phycoerythrin Antibody generated in goat detects Mouse F(ab')2. Representing approximately 75 % of serum immunoglobulins, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.