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Lapin anti-Souris IgG (Heavy & Light Chain) Anticorps (PE) - Preadsorbed

FACS, FM Polyclonal IgG PE F(ab')2 fragment
N° du produit ABIN2669888
  • Antigène Tous les produits IgG
    IgG
    Fragment
    F(ab')2 fragment
    Épitope
    • 2381
    • 512
    • 424
    • 207
    • 132
    • 129
    • 95
    • 29
    • 28
    • 28
    • 22
    • 9
    • 6
    • 6
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    Heavy & Light Chain
    Reactivité
    • 724
    • 701
    • 604
    • 483
    • 403
    • 243
    • 212
    • 176
    • 147
    • 138
    • 113
    • 112
    • 107
    • 102
    • 84
    • 59
    • 48
    • 35
    • 28
    • 16
    • 12
    • 10
    • 8
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Hôte
    • 1792
    • 1575
    • 574
    • 244
    • 112
    • 111
    • 33
    • 31
    • 21
    • 19
    • 15
    • 11
    • 11
    • 1
    Lapin
    Clonalité
    • 3450
    • 178
    • 1
    Polyclonal
    Conjugué
    • 589
    • 418
    • 417
    • 383
    • 282
    • 276
    • 244
    • 119
    • 107
    • 67
    • 66
    • 64
    • 61
    • 58
    • 52
    • 51
    • 48
    • 46
    • 45
    • 44
    • 41
    • 39
    • 36
    • 33
    • 33
    • 31
    • 30
    • 30
    • 29
    • 29
    • 29
    • 29
    • 29
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 28
    • 27
    • 27
    • 26
    • 26
    • 25
    • 22
    • 21
    • 19
    • 17
    • 17
    • 16
    PE
    Application
    • 1956
    • 1852
    • 1810
    • 1522
    • 933
    • 910
    • 652
    • 478
    • 380
    • 236
    • 236
    • 234
    • 106
    • 103
    • 86
    • 67
    • 54
    • 53
    • 31
    • 28
    • 25
    • 21
    • 18
    • 16
    • 11
    • 9
    • 6
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    Specificité
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
     Réactivité croisée
    Souris
    Attributs du produit
    F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Anti-Mouse IgG was produced by repeated immunization with Mouse IgG whole molecule in rabbit.

    Immunogen Type: Native Protein

    Isotype
    IgG
  • Indications d'application

    Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

    Flow Cytometry Dilution: 1:100 - 1:250

    IF Microscopy Dilution: 1:100 - 1:250

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    0.5 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Conseil sur la manipulation
    Product is photosensitive and should be protected from light.
    Stock
    RT,4 °C
    Stockage commentaire
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    Date de péremption
    12 months
  • Antigène
    IgG
    Abstract
    IgG Produits
    Classe de substances
    Antibody
    Sujet

    Synonyms: Rabbit F(ab')2 Anti-Mouse IgG Antibody phycoerythrin Conjugation, Rabbit Fab2 Anti-Mouse IgG PE Conjugated Antibody

    Background: F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate into tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Mouse IgG (H&L) Antibody Phycoerythrin Conjugated Pre-Adsorbed is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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