Souris anti-Humain IgD Anticorps (PE)

N° du produit ABIN2749204

Détail du produit

Antigène: IgD

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: PE

Application: Flow Cytometry (FACS)

Specificité: The mouse monoclonal antibody IA6-2 recognizes human immunoglobulin D.

Réactivité croisée (Details): Human

Purification: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogène: Human IgD

Clone: IA6-2

Isotype: IgG2a kappa

Information d'application

Indications d'application: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Commentaires:

The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Stockage

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Référence

Degauque, Elong Ngono, Ngono, Akl, Lepetit, Crochette, Giral, Lepourry, Pallier, Castagnet, Dugast, Guillot-Gueguen, Jacq-Foucher, Saulquin, Cesbron, Laplaud, Nicot, Brouard, Soulillou: "Characterization of antigen-specific B cells using nominal antigen-coated flow-beads." dans: PLoS ONE, Vol. 8, Issue 12, pp. e84273, (2014) (PubMed).

Bunch, McGregor, Khandoobhai, Aybar, Burkart, Hu, Hogan, Poulton, Berg, Falk, Nachman: "Decreased CD5⁺ B cells in active ANCA vasculitis and relapse after rituximab." dans: Clinical journal of the American Society of Nephrology : CJASN, Vol. 8, Issue 3, pp. 382-91, (2013) (PubMed).

Di Sabatino, Carsetti, Rosado, Ciccocioppo, Cazzola, Morera, Tinozzi, Tinozzi, Corazza: "Immunoglobulin M memory B cell decrease in inflammatory bowel disease." dans: European review for medical and pharmacological sciences, Vol. 8, Issue 5, pp. 199-203, (2005) (PubMed).

Pugh-Bernard, Silverman, Cappione, Villano, Ryan, Insel, Sanz: "Regulation of inherently autoreactive VH4-34 B cells in the maintenance of human B cell tolerance." dans: The Journal of clinical investigation, Vol. 108, Issue 7, pp. 1061-70, (2001) (PubMed).

Ferrari, Giliani, Insalaco, Al-Ghonaium, Soresina, Loubser, Avanzini, Marconi, Badolato, Ugazio, Levy, Catalan, Durandy, Tbakhi, Notarangelo, Plebani: "Mutations of CD40 gene cause an autosomal recessive form of immunodeficiency with hyper IgM." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 22, pp. 12614-9, (2001) (PubMed).

Baier, Baier-Bitterlich, Looney, Altman: "Immunogenic targeting of recombinant peptide vaccines to human antigen-presenting cells by chimeric anti-HLA-DR and anti-surface immunoglobulin D antibody Fab fragments in vitro." dans: Journal of virology, Vol. 69, Issue 4, pp. 2357-65, (1995) (PubMed).

Détail du antigène

Antigène: IgD

Abstract: IgD Produits

Classe de substances: Antibody

Sujet: Immunoglobulin D (IgD) is expressed on the surface of naive mature B cells, thus later than IgM, and is coexpressed with it then. Triggered by antigen binding, it signals through the CD79 complex to activate the B cells. Expression of IgD is lost after the isotype switch. Soluble IgD is present in very small amounts in the serum. IgD can bind to basophils and mast cells to activate them in an IgE-independent way to participate in respiratory immune defense.,Immunoglobulin D