For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions less than 1:100 Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb Le27), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
PBS, pH=7.2, sterile filtered, contains no NaN3
Agent conservateur
Azide free
Conseil sur la manipulation
Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Stock
4 °C
Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, (2000) (PubMed).
The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.