Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 1 μg/10^6 cells. ELISA. Western blotting. Dot- and Slot-Immunoblotting. Immunohistochemistry on Frozen Sections. Immunocytochemistry.
Other applications not tested. Otimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
100 mM Borate Buffered Saline, pH 8.2. No preservatives or amine-containing buffer salts added.
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing.
Stock
4 °C/-20 °C
Stockage commentaire
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
Little is known about the normal function of IgD, and few clinical signs or symptoms are associated with its absence. Individuals with low or absent levels of IgD do not appear unusually predisposed to infections. In mice, IgD may substitute for some functions of IgM when IgM is absent. IgD is expressed on peripheral B cells and is used as a pan B cell marker.Synonyms: Human Immunoglobulin D