ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Specificité
Reacts with the heavy chain of mouse IgG 1 as demonstrated by ELISA and flow cytometry.
Attributs du produit
Source: Pooled antisera from goats hyperimmunized with mouse IgG 1 paraproteins. To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry to conform to characteristics of a standard reference reagent.
Purification
Affinity chromatography on mouse IgG 2b covalently linked to agarose
Working Dilution: 1:2,000-1:4,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions
For Research Use only
Format
Liquid
Conseil sur la manipulation
Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Do not add Sodium azide. Dilute only prior to immediate use Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Conter, Song, Shlomchik, Tomayko: "CD73 expression is dynamically regulated in the germinal center and bone marrow plasma cells are diminished in its absence." dans: PLoS ONE, Vol. 9, Issue 3, pp. e92009, (2014) (PubMed).