ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
Réactivité croisée (Details)
Cross Absorption: Pooled mouse sera and purified mouse paraproteins.
Attributs du produit
Source: Pooled antisera from goats hyperimmunized with human myeloma proteins with ? light chains To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry to conform to characteristics of a standard reference reagent.
Purification
Affinity chromatography on pooled rat IgM + IgG covalently linked to agarose
Working Dilution: 1:5,000-1:20,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Volume d'échantillon
2 mL
Restrictions
For Research Use only
Conseil sur la manipulation
Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Schwaigerlehner, Mayrhofer, Diem, Steinfellner, Fenech, Oostenbrink, Kunert: "Germinality does not necessarily define mAb expression and thermal stability." dans: Applied microbiology and biotechnology, (2019) (PubMed).
Nika, Wallner, Palmberger, Koczka, Vorauer-Uhl, Grabherr: "Expression of full-length HER2 protein in Sf9 insect cells and its presentation on the surface of budded virus-like particles." dans: Protein expression and purification, Vol. 136, pp. 27-38, (2017) (PubMed).