ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Specificité
Reacts with the heavy chain of mouse IgG 2b as demonstrated by ELISA and flow cytometry.
Attributs du produit
Source: Pooled antisera from goats hyperimmunized with mouse IgG 2b paraproteins. To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Purification
Affinity chromatography on mouse IgG 3 covalently linked to agarose.
Working Dilution: 1:4,000-1:8,000 Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions
For Research Use only
Format
Liquid
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C
Jin, Nesbitt, Yang, Chen, Horowitz, Jones, Vandergaast, Carey, Reiter, Russell, Kyratsous, Hooper, Hamilton, Ferreira, Deng, Straus, Baras, Hillyer, Luchsinger: "Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and "Reopening"." dans: PloS one, Vol. 16, Issue 4, pp. e0250319, (2021) (PubMed).
Nesbitt, Jin, Hogan, Yang, Chen, Chan, Simon, Vargas, King, Huard, Bandy, Hillyer, Luchsinger: "Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats." dans: BMC infectious diseases, Vol. 21, Issue 1, pp. 871, (2021) (PubMed).