ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Specificité
Reacts with the heavy chain of BALB/c mouse IgE as demonstrated by ELISA.
Attributs du produit
To insure lot-to-lot consistency, each batch of product is tested by ELISA for conformance to characteristics of a standard reference reagent. This product is not tested for reactivity to other species IgE. Source: Pooled antisera from goats hyperimmunized with BALB/c mouse IgE paraproteins.
Working Dilution: Enzyme-Linked-Immunosorbent-Assay (ELISA) 1:4,000-1:8,000 Indirect immunostaining in conjunction with mouse IgE monoclonal antibodies. Representative data are included in this product insert.
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions
For Research Use only
Format
Liquid
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C/-20 °C
Stockage commentaire
Store at 2-8 °C or long-term at -20 °C.
Joshi, Adamcakova-Dodd, Jing, Wongrakpanich, Gibson-Corley, Thorne, Salem: "Development of a Poly (lactic-co-glycolic acid) Particle Vaccine to Protect Against House Dust Mite Induced Allergy." dans: The AAPS journal, (2014) (PubMed).