ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
Specificité
Reacts with the heavy and light chains of rabbit IgM and IgG, and with the light chains of rabbit IgA as demonstrated by ELISA and flow cytometry.
Attributs du produit
Source: Pooled antisera from goats hyperimmunized with normal rabbit IgM and IgG. To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance to characteristics of a standard reference reagent.
Purification
Affinity chromatography on pooled rat IgM + IgG covalently linked to agarose
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions
For Research Use only
Format
Liquid
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C
Mariot, Wu, Aydin, Mantovani, Mahon, Linglart, Bastepe: "Potent constitutive cyclic AMP-generating activity of XLαs implicates this imprinted GNAS product in the pathogenesis of McCune-Albright syndrome and fibrous dysplasia of bone." dans: Bone, Vol. 48, Issue 2, pp. 312-20, (2011) (PubMed).