Applications: ELISA - Quality tested , FLISA - Quality tested FC - Quality tested , ELISPOT - Reported in literature , WB - Reported in literature , Multiplex - Reported in literature
Working Dilutions: ELISA AP conjugate 1:2,000 - 1:8,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:10,000 - 1:20,000 FLISA FITC conjugate 1:200 - 1:600 AF488 and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells CY5 conjugate 0.3 g/106 cells PE, APC, SPRD, and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg purified immunoglobulin in 1.0 mL of PBS
Agent conservateur
Azide free
Conseil sur la manipulation
Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C,-20 °C
Stockage commentaire
Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt.