Applications: Quality tested applications include - ELISA FLISA FC ,
Other referenced applications include - IHC-FS , IHC-PS , Sep
Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC, TRITC, TXRD, AF488, and AF555 conjugates 1:100 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Stockage commentaire
Store at 2-8°C
Han, Jones, Nicely, Reed, Shen, Mansouri, Louder, Trama, Alam, Edwards, Bonsignori, Tomaras, Korber, Montefiori, Mascola, Seaman, Haynes, Saunders: "Difficult-to-neutralize global HIV-1 isolates are neutralized by antibodies targeting open envelope conformations." dans: Nature communications, Vol. 10, Issue 1, pp. 2898, (2019) (PubMed).
Hammarlund, Thomas, Amanna, Holden, Slayden, Park, Gao, Slifka: "Plasma cell survival in the absence of B cell memory." dans: Nature communications, Vol. 8, Issue 1, pp. 1781, (2018) (PubMed).