Applications: Quality tested applications include - ELISA , FLISA FC ,
Other referenced applications include - ELISPOT , IHC-FS , WB , Sep , Stim
Working Dilutions: ELISA AP conjugate 1:2,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC and AF555 conjugates 1:100 - 1:400 PE conjugate 1 g/mL Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Restrictions
For Research Use only
Buffer
0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Stockage commentaire
Store at 2-8°C
Brandsma, Bondza, Evers, Koutstaal, Nederend, Jansen, Rösner, Valerius, Leusen, Ten Broeke: "Potent Fc Receptor Signaling by IgA Leads to Superior Killing of Cancer Cells by Neutrophils Compared to IgG." dans: Frontiers in immunology, Vol. 10, pp. 704, (2019) (PubMed).
Cheng, Yang, Chen, Hsu, Jou, Hsieh, Tseng: "CLEC9A modulates macrophage-mediated neutrophil recruitment in response to heat-killed Mycobacterium tuberculosis H37Ra." dans: PLoS ONE, Vol. 12, Issue 10, pp. e0186780, (2017) (PubMed).
Trinklein, Pham, Schellenberger, Buelow, Boudreau, Choudhry, Clarke, Dang, Harris, Iyer, Jorgensen, Pratap, Rangaswamy, Ugamraj, Vafa, Wiita, van Schooten, Buelow, Force Aldred: "Efficient tumor killing and minimal cytokine release with novel T-cell agonist bispecific antibodies." dans: mAbs, Vol. 11, Issue 4, pp. 639-652 (PubMed).