Applications: Quality tested applications include - ELISA , FLISA FC ,
Other referenced applications include - ELISPOT , IHC-FS , Stim
Working Dilutions: ELISA BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC conjugate 1:100 - 1:400 AF555 conjugate 1:200 - 1:400 PE and AF647 conjugates 1 g/mL Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE and AF647 conjugates 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Restrictions
For Research Use only
Buffer
0.25 mg in 1.0 mL of PBS/Sodium azide and a stabilizing agent
Agent conservateur
Sodium azide
Précaution d'utilisation
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Conseil sur la manipulation
Do not freeze! Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Stockage commentaire
Store at 2-8°C
Brandsma, Bondza, Evers, Koutstaal, Nederend, Jansen, Rösner, Valerius, Leusen, Ten Broeke: "Potent Fc Receptor Signaling by IgA Leads to Superior Killing of Cancer Cells by Neutrophils Compared to IgG." dans: Frontiers in immunology, Vol. 10, pp. 704, (2019) (PubMed).
Magri, Comerma, Pybus, Sintes, Lligé, Segura-Garzón, Bascones, Yeste, Grasset, Gutzeit, Uzzan, Ramanujam, van Zelm, Albero-González, Vazquez, Iglesias, Serrano, Márquez, Mercade, Mehandru, Cerutti: "Human Secretory IgM Emerges from Plasma Cells Clonally Related to Gut Memory B Cells and Targets Highly Diverse Commensals." dans: Immunity, Vol. 47, Issue 1, pp. 118-134.e8, (2017) (PubMed).