Applications: FC - Quality tested , ELISA - Quality tested , FLISA - Quality tested IHC-FS - Reported in literature , ICC , WB , Multiplex - Reported in literature
Working Dilutions: Flow Cytometry FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells AF647 conjugate 0.1 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L ELISA AP conjugate 1:1,000 - 1:4,000 HRP conjugate 1:4,000 - 1:8,000 BIOT conjugate 1:5,000 - 1:20,000 FLISA FITC and AF488 conjugates 1:200 - 1:400 AF555 conjugate 1:50 - 1:200 PE and AF647 conjugates 1 g/mL
Restrictions
For Research Use only
Concentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Agent conservateur
Without preservative
Conseil sur la manipulation
Each reagent is stable for the period shown on the bottle label if stored as directed.
Stock
4 °C
Stockage commentaire
Store at 2-8°C
Brummelman, Helm, Hamstra, van der Ley, Boog, Han, van Els: "Modulation of the CD4(+) T cell response after acellular pertussis vaccination in the presence of TLR4 ligation." dans: Vaccine, (2015) (PubMed).