ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
Specificité
Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
Sensibilité
Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)
Attributs du produit
IGHE, chromosome 14q32.33
Purification
Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck
Pureté
> 85 %, tested by analytical Gel Permeation Chromatography
When used for functional cellular assays at dilutions < 1:100, the Na-azide free formulation (Cat. No. 0910-1-010 / 050-B) is recommended
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
PBS, pH=7.2, 0.1 % NaN3
Agent conservateur
Sodium azide
Précaution d'utilisation
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.