The reactivity of the antiserum is directed to the Fc subunit of subclass IgG1.
Attributs du produit
Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. Antibody titre: Precipitin titre not less then 1:16 when tested against pooled normal guinea pig serum in agar block immu
Purification
Delipidated, heat inactivated, lyophilized whole antiserum. Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other components of the immunoglobulin system or reacting with other serum proteins. Special attention is given to the removal of antibodies to common Ig/Fab. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Immunogène
Pools of purified normal IgG1 isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in guinea pig serum and other body fluids or to determine its concen-tration.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.
Buffer
No foreign proteins added.
Agent conservateur
Without preservative
Conseil sur la manipulation
Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4 °C, not refrozen, and preferably used the same day.
Stock
4 °C/-20 °C
Stockage commentaire
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.