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Souris anti-Humain IgM Anticorps (TRITC)

IF Monoclonal NI 179 IgG1 kappa TRITC
N° du produit ABIN457907
  • Antigène Tous les produits IgM
    IgM
    Reactivité
    • 226
    • 159
    • 89
    • 42
    • 34
    • 34
    • 28
    • 24
    • 17
    • 14
    • 13
    • 13
    • 13
    • 12
    • 11
    • 4
    • 2
    • 1
    Humain
    Hôte
    • 365
    • 231
    • 88
    • 21
    • 14
    • 5
    • 4
    • 1
    • 1
    Souris
    Clonalité
    • 534
    • 94
    Monoclonal
    Conjugué
    • 122
    • 86
    • 84
    • 66
    • 34
    • 31
    • 26
    • 24
    • 14
    • 12
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 11
    • 10
    • 9
    • 9
    • 9
    • 7
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    TRITC
    Application
    • 377
    • 283
    • 261
    • 221
    • 130
    • 123
    • 91
    • 91
    • 64
    • 62
    • 53
    • 53
    • 45
    • 38
    • 30
    • 20
    • 18
    • 12
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunofluorescence (IF)
    Specificité
    The reactivity of the antiserum is restricted to a isotype specific determinant of the IgM molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunofluorescence staining of cytoplasmic Ig. Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
    Attributs du produit
    Tetramethylrhodamine isothiocyanate-conjugated purified monoclonal mouse antibody to human IgM, isotype specific
    Fluorochrome/IgG protein molar ratio (E/P) approximately 5.7.
    Purification
    Purified
    Immunogène
    Highly purified IgM isolated from human serum.
    Clone
    NI 179
    Isotype
    IgG1 kappa
  • Indications d'application
    To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgM and cell membrane IgM.
    The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100. These data should be interpreted as general recommendations only.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.
    Concentration
    0.4 mg/mL
    Buffer
    Purified monoclonal mouse IgG1 kappa conjugated with TRITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).
    Agent conservateur
    Without preservative
    Conseil sur la manipulation
    Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Antigène
    IgM
    Abstract
    IgM Produits
    Classe de substances
    Antibody
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