Souris anti-Humain IgG3 (Allotype G3m(U)) Anticorps

N° du produit ABIN457932

Détail du produit

Antigène: IgG3

Épitope: Allotype G3m(U)

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Application: ELISA, Immunofluorescence (IF)

Specificité: Monoclonal Mouse antibody to Human IgG3m(u), allotype specific.

Réactivité croisée (Details): The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

Attributs du produit: The reactivity of the antiserum is restricted to an allotype specific determinant of the IgG3m(u) molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3m(u) in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3m(u), and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Purification: Delipidated, heat inactivated, stable ascites

Stérilité: Heat-inactivated

Immunogène: Highly purified monoclonal IgG3m(u) isolated from human serum

Clone: NI 86 (HP 6080)

Isotype: IgG1

Information d'application

Indications d'application: Indirect immunofluorescence,ELISA.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/mL. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Buffer: Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/mL. No foreign proteins added.

Agent conservateur: Without preservative

Stock: RT,4 °C,-20 °C

Stockage commentaire: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.

Détail du antigène

Antigène: IgG3

Abstract: IgG3 Produits

Classe de substances: Antibody