Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
Specificité
The defined antibody specificity is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibody to common determinants and to class specific determinants and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). In immuno-electrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA, IgM and IgD (if present in precipitable amounts) precipitin lines are obtained.
Attributs du produit
Purified IgG fraction of polyclonal goat antiserum to human immunoglobulins IgG, IgA, IgM and IgD, heavy and light chains
Purification
Preadsorption: Immunoaffinity adsorbed using insolubilized antigens.
Immunogène
Highly purified IgG, and pools of homogenous IgA, IgM and IgD isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
The cytochemical grade of GAHu/Ig (GAMD) /7S allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250, in ELISA and comparable non-precipitating antibody-binding assays are between 1:1,000 and 1:5,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
Concentration
10 mg/mL
Buffer
Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
Agent conservateur
Without preservative
Conseil sur la manipulation
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Stock
4 °C/-20 °C
Stockage commentaire
The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.