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Lapin anti-Souris IgG2 Anticorps (FITC)

IF Polyclonal IgG FITC
N° du produit ABIN458530
  • Antigène Tous les produits IgG2
    IgG2
    Reactivité
    • 22
    • 12
    • 7
    • 7
    • 6
    • 1
    • 1
    Souris
    Hôte
    • 20
    • 11
    • 10
    • 9
    • 6
    Lapin
    Clonalité
    • 30
    • 26
    Polyclonal
    Conjugué
    • 21
    • 9
    • 8
    • 7
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 30
    • 28
    • 25
    • 21
    • 14
    • 8
    • 7
    • 7
    • 6
    • 4
    • 3
    • 2
    • 2
    • 1
    Immunofluorescence (IF)
    Specificité
    The reactivity of the antiserum is directed to the subclasses IgG2a and IgG2b as tested against mouse sera and a panel of purified homogenous immunoglobulins.
    Attributs du produit
    Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal rabbit antiserum to mouse IgG2, subclass specific
    Fluorochrome/IgG protein molar ratio (F/P) is approximately 2.0.
    Purification
    Undesired traces of antibody activity are eliminated by immunoaffinity chromatography as required. Hyperimmune antisera with strong precipitating activity are selected for fractionation by salt-precipitation and purification of the IgG fraction by DEAE-chromatography.
    Immunogène
    Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.
    Isotype
    IgG
  • Indications d'application
    To identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen or immune complex using a reference antibody of mouse origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted.
    The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40.
    Commentaires

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -24 °C.
    Concentration
    10 mg/mL
    Buffer
    Fluorochrome-coupled purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2).
    Agent conservateur
    Without preservative
    Conseil sur la manipulation
    Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, a nd preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
    Stock
    4 °C/-20 °C
    Stockage commentaire
    The lyophilized conjugate is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.
  • Antigène
    IgG2
    Abstract
    IgG2 Produits
    Classe de substances
    Antibody
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