Chèvre anti-Boeuf (Vache) IgG (Heavy & Light Chain) Anticorps (HRP)

N° du produit ABIN5608132

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Boeuf (Vache)

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Attributs du produit: Bovine IgG (H&L) Antibody Peroxidase Conjugated detects Bovine Immunoglobulin G. Immunoglobulin G, IgG, are antibody molecules. Each IgG is a hetereotetramer composed of four peptide chains - two heavy chains and two light chains. Each IgG has two F(ab) antigen binding sites.

Purification: Anti-Bovine IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Bovine IgG and Bovine Serum.

Immunogène:

Immunogen: Bovine IgG whole molecule

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:500 - 1:5,000

Application Note: Anti-Bovine IgG (heavy and light chain) Antibody is peroxidase conjugated and is suitable for immunoassays where specificity to bovine immunoglobulin is desired. Antibody has been tested by ELISA, western blot immunoblot, and immunohistochemistry. Optimal concentrations in immunoassays should be determined by the researcher.

ELISA Dilution: 1:5,000 - 1:100,000

Western Blot Dilution: 1:10,000 - 1:200,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.8 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 15 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: None. Do NOT add sodium azide!

Agent conservateur: Without preservative

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: goat anti-Bovine IgG peroxidase Conjugated Antibody, goat anti-Bovine IgG HRP Conjugated Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. This product possesses the F(ab) region possessing the epitope-recognition site, both heavy and light chains of the antibody molecule are present. Bovine IgG (H&L) Antibody Peroxidase Conjugated detects Bovine Immunoglobulin G. Immunoglobulin G, IgG, are antibody molecules. Each IgG is a hetereotetramer composed of four peptide chains - two heavy chains and two light chains. Each IgG has two F(ab) antigen binding sites.