Lapin anti-Souris IgG Anticorps (HRP) - Preadsorbed

N° du produit ABIN5608149

Détail du produit

Antigène: IgG

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Attributs du produit: This Anti-Mouse IgG subclass pan reactive Secondary Antibody is is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development.

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1000 - 1:5000

Application Note: This Anti-Mouse IgG subclass pan reactive Secondary Antibody is designed for Western Blotting, ELISA and Immunohistochemistry. HRP conjugated secondary antibodies can also be used for a variety of other applications such as Assay Development. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.

ELISA Dilution: 1:20,000 - 1:40,000

Western Blot Dilution: 1:2000 - 1:10000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody

Sujet:

Synonyms: rabbit anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) Antibody peroxidase conjugation, rabbit anti-Mouse IgG HRP conjugated Antibody

Background: Anti-Mouse IgG Peroxidase Antibody generated in rabbit detects reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsinization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. IgG1, IgG2a, IgG2b and IgG3 chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.