Chèvre anti-Rat IgA (Heavy Chain) Anticorps (Biotin) - Preadsorbed

N° du produit ABIN5608175

Détail du produit

Antigène: IgA

Épitope: Heavy Chain

Reactivité: Rat

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Rat IgA alpha heavy chain

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: This product has been assayed against 1.0 μg of Rat IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:30,000 of the reconstitution concentration is suggested for this product.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détail du antigène

Antigène: IgA

Abstract: IgA Produits

Classe de substances: Antibody

Sujet:

Synonyms: Goat anti-Rat IgA (alpha chain) biotin Conjugated Antibody, Goat anti-Rat IgA alpha Antibody biotin Conjugation

Background: Anti-Rat IgA Antibody generated in goat detects immunoglobulin A alpha chain from rat. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. This Anti-Rat IgA is conjugated to Biotin