Âne anti-Humain IgM (Fc5mu Region) Anticorps (PE) - Preadsorbed

N° du produit ABIN5608181

Détail du produit

Antigène: IgM

Fragment: F(ab')2 fragment

Épitope: Fc5mu Region

Reactivité: Humain

Hôte: Âne

Clonalité: Polyclonal

Conjugué: PE

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Purification: Preadsorption: Solid phase absorption

Immunogène:

Immunogen: Human IgM Fc5mu  fragment

Isotype: IgG

Information d'application

Indications d'application:

Application Note: F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:50 to 1:200 should be suitable for most applications.

Flow Cytometry Dilution: 1:100 - 1:250

IF Microscopy Dilution: 1:100 - 1:250

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 0.5 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: RT,4 °C

Stockage commentaire: Store vial at 4° C prior to opening. Dilute only prior to immediate use. Do not freeze after reconstitution. Store reagent in the dark. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis.

Date de péremption: 6 months

Détail du antigène

Antigène: IgM

Abstract: IgM Produits

Classe de substances: Antibody

Sujet:

Synonyms: Donkey F(ab')2 anti-Human IgM Fc5u Antibody Phycoerythrin Conjugation, Donkey F(ab')2 anti-Human IgM Fc5u PE Conjugated Antibody

Background: F(ab')2 Anti-Human IgM Fc5μ Phycoerythrin Antibody generated in donkey detects specifically the Fc5μ portion of the human IgM heavy chain. Immunoglobulin M is the largest antibody isotype and the first to be secreted against an initial exposure to antigen. IgM is predominantly produced in the spleen. Formed from covalently linking 5 immunoglobulins together. F(ab')2 Anti-Human IgM Fc5μ antibody is ideal for investigators in Immunology, Microbiology, and Cell Biology.