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Lapin anti-Souris lambda (Light Chain) Anticorps (Biotin) - Preadsorbed

ELISA, IHC, WB Polyclonal IgG Biotin
N° du produit ABIN5608187
  • Antigène
    lambda
    Épitope
    • 18
    • 10
    Light Chain
    Reactivité
    • 27
    • 13
    • 4
    Souris
    Hôte
    • 26
    • 8
    • 6
    • 4
    Lapin
    Clonalité
    • 32
    • 12
    Polyclonal
    Conjugué
    • 8
    • 8
    • 7
    • 7
    • 4
    • 4
    • 3
    • 3
    Biotin
    Application
    • 33
    • 31
    • 11
    • 11
    • 9
    • 9
    ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
    Purification
    Preadsorption: Solid phase absorption
    Immunogène

    Immunogen: Mouse lambda light chain

    Isotype
    IgG
  • Indications d'application

    Immunohistochemistry Dilution: 1:1,000 - 1:5,000

    Application Note: This product has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:10,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    ELISA Dilution: 1:20,000 - 1:100,000

    Western Blot Dilution: 1:2,000 - 1:10,000

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Concentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    Agent conservateur
    Sodium azide
    Précaution d'utilisation
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Date de péremption
    12 months
  • Antigène
    lambda
    Autre désignation
    lambda )
    Sujet

    Synonyms: Rabbit Anti-Mouse λ (lambda chain) Biotin Conjugated Antibody, Rabbit Anti Mouse lambda Antibody Biotin conjugation

    Background: Anti-Mouse λ (lambda chain) (RABBIT) Antibody generated in rabbit detects specifically Mouse lambda light chain. Immunoglobulins are heterotetramers composed of 2 immunoglobulin heavy and 2 immunoglobulin light chains. The immunoglobulin light chain is the small polypeptide subunit of an antibody (immunoglobulin). The light chains can be categorized into kappa type or lambda type and both are used to construct the antigen binding F(ab) region of an antibody along with the variable region of the heavy chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. Anti-Mouse IgG lambda is conjugated to biotin.

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