Immunoprecipitation (IP), Immunofluorescence (IF), Dot Blot (DB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunodiffusion (ID), Enzyme Immunoassay (EIA), Western Blotting (WB)
Attributs du produit
Molar Ratio: Peroxidase/IgG aprox. 1.0
Purification
Hyperimmune antisera with strong precipitating activity are selected for fractionation and purification of the IgG (7S) fraction containing the bulk of the defined antibody specificity. It is free of other serum proteins as tested by immunoelectrophoresis.
Immunogène
Pools of purified homogenous IgG2a and IgG2b isolated from rat sera. Freund's complete adjuvant is used in the first step of the immunization procedure.
Optimal working dilutions should be determined experimentally by the investigator.
Restrictions
For Research Use only
Format
Liquid
Reconstitution
Restore with 1,0 mL distilled water. Working dilutions should be prepared by adding sterile PBS, pH 7.2
Concentration
10.0 mg/mL
Buffer
PBS, pH 7.2 without preservatives and foreign proteins
Agent conservateur
Without preservative
Conseil sur la manipulation
Avoid repeated freezing and thawing. Dilute only prior to immediate use Centrifuge product if not completely clear after standing at room temperature. Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Stock
4 °C/-20 °C
Stockage commentaire
Prior to and following reconstitution store the antibody at 2-8 °C for one month or at -28 °C for longer.