Chèvre anti-Lapin IgG (Heavy & Light Chain) Anticorps (DyLight 550)

N° du produit ABIN612393

Détail du produit

Antigène: IgG

Épitope: Heavy & Light Chain

Reactivité: Lapin

Hôte: Chèvre

Conjugué: DyLight 550

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Attributs du produit: Goat anti-rabbit IgG (H&L) - Affinity Pure, DyLight 550 Conjugate.
Fluorphore: DyLight 550 (Ex = 550 nm, Em = 576 nm).
Fluor Protein Ratio: Moles DyLight 550 per Mole Antibody.

Purification: Affinity purified using solid phase human IgM (H&L)

Pureté: > 95 % based on SDS-PAGE

Immunogène: Purified rabbit IgG, whole molecule

Information d'application

Indications d'application: This conjugate is suitable for immunomicroscopy, flow cytometry.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:20 - 1:2,000 for most applications

Commentaires:

Country of Origin: Goat serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. 0.05 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Stock: 4 °C

Référence

Michell, Chen Zp, Tiganis, Stapleton, Katsis, Power, Sim, Kemp: "Coordinated control of endothelial nitric-oxide synthase phosphorylation by protein kinase C and the cAMP-dependent protein kinase." dans: The Journal of biological chemistry, Vol. 276, Issue 21, pp. 17625-8, (2001) (PubMed).

Dimmeler, Fleming, Fisslthaler, Hermann, Busse, Zeiher: "Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation." dans: Nature, Vol. 399, Issue 6736, pp. 601-5, (1999) (PubMed).

Gallis, Corthals, Goodlett, Ueba, Kim, Presnell, Figeys, Harrison, Berk, Aebersold, Corson: "Identification of flow-dependent endothelial nitric-oxide synthase phosphorylation sites by mass spectrometry and regulation of phosphorylation and nitric oxide production by the phosphatidylinositol 3-kinase inhibitor LY294002." dans: The Journal of biological chemistry, Vol. 274, Issue 42, pp. 30101-8, (1999) (PubMed).

Détail du antigène

Antigène: IgG

Abstract: IgG Produits

Classe de substances: Antibody