1:500-1: 5000 for immunohistochemistry (IHC),1:10 000-1:50 000 for ELISA/western blot-
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
For reconstitution add 1.1 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.
Conseil sur la manipulation
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming. A solution with 50 % glycerol will not freeze in -20 °C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol.
Stock
4 °C
Stockage commentaire
Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1.1 ml of sterile water add 1.1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.