Chèvre anti-Humain IgA, IgG, IgM Anticorps (DyLight 800) - Preadsorbed
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- Antigène Tous les produits IgA, IgG, IgM
- IgA, IgG, IgM
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Reactivité
- Humain
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Hôte
- Chèvre
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Clonalité
- Polyclonal
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Conjugué
- DyLight 800
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Fluorescence Microscopy (FM), FLISA
- Attributs du produit
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Synonyms: Goat anti Human IgG IgA IgM Antibody DyLight 800™ Conjugation, Goat Anti-Human IgG IgA IgM DyLight 800™ Conjugation Antibody, Goat Anti-Human IgGAM DyLight 800™ Conjugation
Background: Anti-Human IgG IgA IgM (H&L) DyLight 800 Antibody generated in goat detects human (heavy and light chain) immunoglobulin G, A, and M. Immunoglobulin G binds to antigens and can neutralize or opsinize targets, and are predominantly involved in the secondary immune response. Immunoglobulin A (IgA) is an antibody that plays a critical role in mucosal immunity. IgA has two subclasses (IgA1 and IgA2) and can exist in a dimeric form called secretory IgA (sIgA). Immunoglobulin M, or IgM, is a pentamer composed of 5 immunoglobulin molecules linked through their F(c) domain by the J chain. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Purification
- Preadsorption: Solid phase absorption
- Immunogène
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Immunogen: Human IgG, IgA, and IgM whole molecules
- Isotype
- IgG
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- Indications d'application
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Immunohistochemistry Dilution: 1:1,000 - 1:5,000
Application Note: Anti-Human IgG IgA IgM (H&L) DyLight 800 Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
ELISA Dilution: 1:70,000
Western Blot Dilution: 1:2,000 - 1:10,000
IF Microscopy Dilution: >1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Date de péremption
- 12 months
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- Antigène
- IgA, IgG, IgM
- Autre désignation
- iga,igg,igm (IgA, IgG, IgM Produits)
- Classe de substances
- Antibody
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