Lapin anti-Souris IgG2a (Chain gamma 2a) Anticorps (DyLight 488) - Preadsorbed
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- Antigène Tous les produits IgG2a
- IgG2a
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Épitope
- Chain gamma 2a
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Reactivité
- Souris
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Hôte
- Lapin
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Clonalité
- Polyclonal
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Conjugué
- DyLight 488
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Application
- Western Blotting (WB), FLISA, Fluorescence Microscopy (FM)
- Attributs du produit
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Synonyms: Rabbit Anti-Mouse IgG2a (Gamma 2a chain) Antibody DyLight 488™ Conjugated, Rabbit Anti Mouse IgG2a DyLight 488™ Conjugated Antibody
Background: Anti-Mouse IgG2a DyLight 488 Antibody generated in rabbit detects reactivity to Mouse IgG2a (Gamma 2a chain). Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. IgG2, the second largest of IgG isotypes, comprises almost 25 % of IgG and has a low affinity for binding to the Fc receptor of phagocytic cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
- Purification
- Preadsorption: Solid phase absorption
- Immunogène
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Immunogen: Mouse IgG2a heavy chain
- Isotype
- IgG
- Labeling Ratio
- 3.1
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- Indications d'application
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
FLISA Dilution: >1:20,000
Western Blot Dilution: >1:10,000
IF Microscopy Dilution: >1:5,000
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
0.01 % (w/v) Sodium Azide - Agent conservateur
- Sodium azide
- Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Stock
- RT,4 °C,-20 °C
- Stockage commentaire
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Date de péremption
- 12 months
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- Antigène
- IgG2a
- Abstract
- IgG2a Produits
- Classe de substances
- Antibody
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